Cell Line Authentication
Cell Line Authentication is an essential research tool that finds its use in medicine, genetics, vaccine development, cell biology, etc. A lot of times, cell lines are misidentified or contaminated with some other cells. Therefore, authentication of the cell line is essential every time a new line is acquired or established. STR analysis is one of the most reliable methods of Cell Line Authentication DNA testing.
A cell line is a generic term for a population of cells maintained in cell culture for an extended time. Cell Line Identification verifies the identity of a cell line and matches it with the available standard database of cell lines. We use standardized authentication methods to detect genetically drifted, misidentified, or cross-contaminated cells. The research analysis requires using a model that helps generate repeated DNA for different human cell lines.
It is advisable to select a NABL-accredited DNA testing company for Cell Line Authentication.
Cell Line Authentication Process
Cell Line Authentication analyses the STRs (short tandem repeats), starting with the amplification process. At least 8 STR loci are used as per the process for genetic profile authentication.
The STR DNA typing generates reproducible data following the guidelines of the important scientific units.
The steps involved in Cell Line Authentication:
1. Generate an STR profile by multiplex PCR
2. Perform capillary electrophoresis in a genetic analyzer
3. Analyze the data obtained by the GMIDX software
When is the right time to execute Cell Line Authentication?
It is the right time to carry out Cell Line Authentication,
• Before starting a new experiment
• At early stages to determine cell line identity
• Before research publication, when the culture is growing
• When you are unable to reproduce the results of the experiment, or the cell line behavior is not as per the expectation
• When passaging cell lines as a practice
Cell Line Authentication extensively finds its use in pharmaceutical companies, research institutes, and biotechnology companies. DNA Forensics Laboratory Pvt. Ltd. facilitates fast, easy, and reliable STR profiling of cell lines. We use the best methods for the cross-examination of cells and misidentification of cells.
